Label-free plasmonic-based biosensing using a gold nanohole array chip coated with a wafer-scale deposited WS2 monolayer

Lixing Kang (1) , Yan Zhang (2, 3) , Qian Gong (4) , Chandreyee Manas Das (1) , Huilin Shao (3) , Daniel Puiu Poenar (1) , Philippe Coquet (5, 1) , Ken-Tye Yong (6)

1 CINTRA - CNRS International - NTU - Thales Research Alliance
2 Jiangnan University
3 NUS - National University of Singapore
4 CAS - Chinese Academy of Sciences [Beijing]
5 IEMN - Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
6 EEE - School of Electrical and Electronic Engineering

This paper reports the fabrication, testing and obtained performance of a plasmonic sensor employing a gold (Au) nanohole array chip coated with tungsten disulphide (WS2), which is then functionalized for the detection of protein–protein interactions. A key novelty is that the WS2 was deposited as a monoatomic layer using a wafer-scale synthesis method that successfully provided a film of both high quality and uniform thickness. The deposited WS2 film was transferred onto a Au nanohole array chip using a novel method and was subsequently functionalized with biotin. The final sensor was tested and it demonstrated efficient real-time and label-free plasmonic detection of biotin–streptavidin coupling. Specifically, compared to a standard (i.e. uncoated) Au nanohole-based sensor, our WS2-coated Au nanohole array boosted the spectral shift of the resonance wavelength by ∼190%, resulting in a 7.64-fold improvement of the limit of detection (LOD).

Domaines: Sciences de l'ingénieur [physics]

Lixing Kang, Yan Zhang, Qian Gong, Chandreyee Manas Das, Huilin Shao, et al.. Label-free plasmonic-based biosensing using a gold nanohole array chip coated with a wafer-scale deposited WS2 monolayer. RSC Advances, 2022, 12 (51), pp.33284-33292. ⟨10.1039/D2RA03479D⟩. ⟨hal-03869405⟩